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KMID : 0364820090450010063
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Korean Journal of Microbiology
2009 Volume.45 No. 1 p.63 ~ p.68
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Analysis of β-(1→3)(1→6)-Glucan Produced by Aureobasidium pullulans IMS-822
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Lee Seog-June
Ahn Kuk-Hyun
Park Chan-Sun
Yoon Byeong-Dae
Kim Min-Soo
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Abstract
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The exo-polysaccharide producing microorganism, Aureobasidium pullulans IMS-822, was isolated and identified from soil. The viscosity-average molecular weight of exo-polysaccharide was calculated as 8.9×105 by Mark-Houwink equation. The sugar component of exo-polysaccharide was determined as glucose by HPLC analysis. The IR spectra indicated that the exo-polysaccharide has an absorption peak at 890 cm-1 for the β-configuration of D-glucan. The 13C NMR signal at δ 86.62 ppm arose from the substituted C-3 of glucose. The signal at δ 72.11 ppm was assigned to C-6 of branched β-(1→3)-D-glucosyl residues. Viscosity and Congo red reaction indicated that β-(1→3)(1→6)-glucan produced by A. pullulans IMS-822 has a highly ordered hydrogen- bond dependent conformation in aqueous solution, which collapses in strong alkaline solution.
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KEYWORD
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Aureobasidium sp., β-(1→3)(1→6)-glucan, viscosity-average molecular weight, aqueous conformation
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